5O50
Glycogen Phosphorylase b in complex with 33a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2016-09-06 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.04 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.480, 128.480, 116.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.860 - 1.900 |
| R-factor | 0.13197 |
| Rwork | 0.130 |
| R-free | 0.16098 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.329 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.860 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 76499 | 10969 |
| <I/σ(I)> | 12 | 3.2 |
| Completeness [%] | 99.6 | 99.2 |
| Redundancy | 4.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 6.8 | 289 | 10 mM BES buffer |
