5O47
Structure of D80A-fructofuranosidase from Xanthophyllomyces dendrorhous complexed with fructosyl-hydroxytyrosol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97926 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 74.616, 205.356, 146.940 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.980 - 1.910 |
R-factor | 0.16854 |
Rwork | 0.168 |
R-free | 0.18213 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 5ann |
RMSD bond length | 0.006 |
RMSD bond angle | 1.413 |
Data reduction software | XDS |
Data scaling software | Aimless |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.980 | 1.940 |
High resolution limit [Å] | 1.910 | 1.910 |
Rmerge | 0.072 | |
Rpim | 0.030 | |
Number of reflections | 175297 | |
<I/σ(I)> | 19 | |
Completeness [%] | 100.0 | |
Redundancy | 6.8 | |
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | Protein was crystallized from 1.3M Sodium Citrate tribasic dihydrate |