5O3O
Pronase-treated paired helical filament in Alzheimer's disease brain
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Quantum |
| Detector | GATAN K2 QUANTUM (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 3.5 |
| Number of Particle | 66585 |
| Software | RELION,Gctf,Coot,REFMAC |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
