5O35

Structure of complement proteins complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-01
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.987
Spacegroup name	P 31 2 1
Unit cell lengths	142.900, 142.900, 312.721
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	61.878 - 4.200
R-factor	0.2175
Rwork	0.216
R-free	0.24610
RMSD bond length	0.004
RMSD bond angle	0.891
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	61.880
High resolution limit [Å]	4.200
Rmerge	0.089
Rpim	0.089
Number of reflections	27699
<I/σ(I)>	7.4
Completeness [%]	99.9
Redundancy	2
CC(1/2)	0.994

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	60mM Ammonium sulfate, 6% w/v PEG3350