5O2W
Extended catalytic domain of Hypocrea jecorina LPMO 9A.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-20 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.872900 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.936, 62.125, 48.015 |
| Unit cell angles | 90.00, 111.79, 90.00 |
Refinement procedure
| Resolution | 44.580 - 2.000 |
| R-factor | 0.2021 |
| Rwork | 0.200 |
| R-free | 0.23820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.739 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | |
| High resolution limit [Å] | 2.000 | |
| Number of reflections | 21394 | |
| <I/σ(I)> | 4.6 | 2 |
| Completeness [%] | 94.2 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.6 M ammonium sulfate and 0.1 M citric acid pH 4.0 |
