5O2U
Llama VHH in complex with p24
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.976251 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.993, 72.228, 69.953 |
| Unit cell angles | 90.00, 91.40, 90.00 |
Refinement procedure
| Resolution | 36.110 - 2.760 |
| R-factor | 0.224 |
| Rwork | 0.222 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hgp and 4ldo |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.578 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.110 | 2.910 |
| High resolution limit [Å] | 2.760 | 2.760 |
| Rmerge | 0.142 | 0.712 |
| Rpim | 0.098 | 0.473 |
| Number of reflections | 10360 | 1508 |
| <I/σ(I)> | 5.55 | 1.7 |
| Completeness [%] | 97.7 | 98.5 |
| Redundancy | 3 | 3 |
| CC(1/2) | 0.984 | 0.572 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 100mM Tris base pH 8.0, Polyethylene glycol (PEG) 6000 25% Ethylene Glycol |
