5O2O
Lytic transglycosylase in action
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-20 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.714, 72.681, 124.924 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.150 - 1.430 |
| R-factor | 0.19199 |
| Rwork | 0.191 |
| R-free | 0.22578 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4YIM |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.591 |
| Data reduction software | XDS |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.150 |
| High resolution limit [Å] | 1.430 |
| Number of reflections | 110458 |
| <I/σ(I)> | 0.185 |
| Completeness [%] | 97.6 |
| Redundancy | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 277.15 | 100 mM CHES pH 9.5, 100 mM tri sodium citrate, 30% PEG 3000 |
