5O2L

Myosin VI motor domain in the Pre-Transition State

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9800
Spacegroup name	P 21 21 21
Unit cell lengths	72.340, 83.830, 177.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.180 - 2.200
R-factor	0.1844
Rwork	0.182
R-free	0.22680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v26
RMSD bond length	0.010
RMSD bond angle	1.060
Data reduction software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.11.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.260
High resolution limit [Å]	2.200	2.200
Number of reflections	55469
<I/σ(I)>	15.57	1.29
Completeness [%]	99.6
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	7% polyethylene glycol [PEG] 8000, 50 mM TRIS, pH 7.5, 1 mM TCEP, 15% glycerol