5O2C
Crystal structure of WNK3 kinase and CCT1 didomain in a unphosphorylated state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 166.370, 64.080, 47.340 |
| Unit cell angles | 90.00, 97.16, 90.00 |
Refinement procedure
| Resolution | 24.470 - 2.400 |
| R-factor | 0.1885 |
| Rwork | 0.185 |
| R-free | 0.25420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.160 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 32.780 |
| High resolution limit [Å] | 2.400 |
| Number of reflections | 19384 |
| <I/σ(I)> | 6.3 |
| Completeness [%] | 99.6 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 18% PEG3350, 0.1 M Citrate pH 5 |
