5O24
Lytic transglycosylase in action
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-05-20 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.714, 72.681, 124.924 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.740 - 1.429 |
R-factor | 0.1915 |
Rwork | 0.191 |
R-free | 0.22400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4YIM |
RMSD bond length | 0.005 |
RMSD bond angle | 0.771 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.740 | 1.480 |
High resolution limit [Å] | 1.429 | 1.429 |
Rmerge | 0.185 | 0.250 |
Rpim | 0.064 | 0.880 |
Number of reflections | 110571 | 10064 |
<I/σ(I)> | 14.4 | 0.41 |
Completeness [%] | 97.5 | 90.47 |
Redundancy | 9.3 | 8.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 277.15 | 100 mM CHES pH 9.5, 100 mM tri sodium citrate, 30% PEG 3000 |