5O1Z
Structure of Nrd1 RNA binding domain in complex with RNA (CGUAAA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979290 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 64.529, 64.529, 157.033 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.690 - 3.400 |
| R-factor | 0.18301 |
| Rwork | 0.181 |
| R-free | 0.21585 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5o1w |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.223 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.690 | 3.670 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.157 | 0.578 |
| Rpim | 0.033 | 0.116 |
| Number of reflections | 5025 | |
| <I/σ(I)> | 22.2 | 8.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 23.9 | 25.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 1M Sodium Potassium Phosphate pH 7.4 |
