5O1J
Lytic transglycosylase in action
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.135, 69.122, 143.321 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 71.660 - 1.900 |
| R-factor | 0.2025 |
| Rwork | 0.201 |
| R-free | 0.24906 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4YIM |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.592 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 71.660 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 54011 |
| <I/σ(I)> | 7.81 |
| Completeness [%] | 99.4 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291.15 | 0.2M Zinc acetate, or 0.1 M Sodium cacodylate pH 6.5, 0.1M 18%PEG 8000 |
