5O10
Y48H mutant of human cytochrome c
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-11 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.999 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.937, 36.498, 60.700 |
Unit cell angles | 90.00, 116.65, 90.00 |
Refinement procedure
Resolution | 54.250 - 1.360 |
R-factor | 0.146 |
Rwork | 0.144 |
R-free | 0.17500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zcf |
RMSD bond length | 0.015 |
RMSD bond angle | 2.179 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.250 | 1.390 |
High resolution limit [Å] | 1.360 | 1.360 |
Rmerge | 0.072 | 1.115 |
Rpim | 0.031 | 0.510 |
Number of reflections | 45812 | 2124 |
<I/σ(I)> | 13.3 | 1.4 |
Completeness [%] | 95.3 | 90.2 |
Redundancy | 6.2 | 5.4 |
CC(1/2) | 0.998 | 0.510 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 33% PEG 6000 100mM Tris pH 7.5 |