5O0I
ADP-dependent glucokinase from Pyrococcus horikoshii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-26 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 77.135, 77.135, 133.889 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.630 - 2.000 |
| R-factor | 0.1709 |
| Rwork | 0.168 |
| R-free | 0.21910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l2l |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.146 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.300 | 2.072 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmeas | 0.264 | 1.835 |
| Number of reflections | 31825 | |
| <I/σ(I)> | 13.44 | 1.9 |
| Completeness [%] | 99.9 | 99.2 |
| Redundancy | 19.78 | 20 |
| CC(1/2) | 0.997 | 0.556 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 15% (w/v) PEG 6000, 0.2 M LiSO4, and 0.1 M citrate buffer (pH 3.6) |
