5O03

GII.10 Vietnam 026 protruding domain in complex with Nanobody Nano-32

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-07
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.965
Spacegroup name	P 41 21 2
Unit cell lengths	109.660, 109.660, 268.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.041 - 2.194
R-factor	0.1732
Rwork	0.172
R-free	0.19290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.873
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.041	49.041	2.330
High resolution limit [Å]	2.190	6.530	2.190
Rmerge	0.059	0.036	0.773
Rmeas	0.062	0.038	0.824
Number of reflections	84293	3518	13258
<I/σ(I)>	23.86	62.98	2.66
Completeness [%]	99.8	99.4	98.9
Redundancy	10.641	9.741	8.486
CC(1/2)	0.999	0.999	0.925

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 M magnesium formate