5NZ6
The structure of the thermobifida fusca guanidine III riboswitch with guanidine in space group P3212.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.92019 |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 83.560, 83.560, 98.814 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.938 - 2.940 |
| R-factor | 0.1924 |
| Rwork | 0.191 |
| R-free | 0.21690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5nwq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.721 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2219: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.410 | 2.990 |
| High resolution limit [Å] | 2.940 | 2.940 |
| Rmerge | 0.101 | 1.658 |
| Rpim | 0.023 | 0.367 |
| Number of reflections | 16177 | 434 |
| <I/σ(I)> | 18.2 | 1.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 21.57 | 21.1 |
| CC(1/2) | 0.965 | 0.964 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 60% v/v Tacsimate pH 7.0 |
