5NYQ
Anbu (Gly-1) mutant from Hyphomicrobium sp. strain MC1 - SG:R32
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 175.548, 175.548, 39.742 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.950 |
| R-factor | 0.1793 |
| Rwork | 0.177 |
| R-free | 0.21414 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5nyf |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.085 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.050 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.041 | 0.584 |
| Number of reflections | 17067 | |
| <I/σ(I)> | 31.5 | 4.27 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298.15 | 0.8 M sodium phosphate, 0.8 M potassium phosphate, 0.1 M HEPES |
