5NYK
Crystal structure of the atypical poplar thioredoxin-like2.1 in oxidized state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2011-08-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.82656 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.697, 52.863, 59.083 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.370 - 1.050 |
| R-factor | 0.155 |
| Rwork | 0.155 |
| R-free | 0.16220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ep7 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.400 | 1.070 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.069 | 0.731 |
| Rpim | 0.280 | 0.295 |
| Number of reflections | 66294 | 3045 |
| <I/σ(I)> | 19.4 | 3.4 |
| Completeness [%] | 95.6 | 89.9 |
| Redundancy | 13.5 | 13.2 |
| CC(1/2) | 0.999 | 0.883 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 0.4M NaH2PO4, 1.6M K2PO4, 0.2M NaCl, imidazole |
