5NY0
L. reuters 100-23 SRRP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2014-04-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9002 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 162.356, 162.356, 146.777 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.969 - 2.000 |
| R-factor | 0.2251 |
| Rwork | 0.224 |
| R-free | 0.24450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5nxk |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.397 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.060 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 37741 | 5451 |
| <I/σ(I)> | 21.2 | 5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.2 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 295 | 0.2 M sodium tartrate dibasic, 20% PEG 3350 |
