5NXT

Wobble base paired RNA double helix

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2016-01-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 63 2 2
Unit cell lengths	44.997, 44.997, 94.205
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.969 - 1.379
R-factor	0.1308
Rwork	0.130
R-free	0.15140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ts0
RMSD bond length	0.010
RMSD bond angle	1.490
Data reduction software	XDS
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.970	1.460
High resolution limit [Å]	1.379	1.380
Number of reflections	12286
<I/σ(I)>	15.08	1.5
Completeness [%]	99.7	99.8
Redundancy	10.47
CC(1/2)	1.000	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	277	PEG 6000, Lithium chloride, Citric Acid