5NXN
Structure of a L5-deletion mutant of Providencia stuartii Omp-Pst1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-31 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.93 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.321, 158.985, 101.326 |
| Unit cell angles | 90.00, 124.65, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.120 |
| R-factor | 0.24735 |
| Rwork | 0.246 |
| R-free | 0.26795 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d64 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.948 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.210 |
| High resolution limit [Å] | 3.110 | 3.110 |
| Rmerge | 0.202 | 1.681 |
| Number of reflections | 29184 | 2342 |
| <I/σ(I)> | 8.16 | 1.03 |
| Completeness [%] | 97.3 | 79.2 |
| Redundancy | 6.78 | 6.1 |
| CC(1/2) | 0.998 | 0.605 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 25% PEG 600, 0.2 M NaCl, 0.1 M MES pH 6.5 |
