5NXK
L. reuteri 53608 SRRP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2014-03-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 146.697, 146.697, 110.423 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.770 - 1.918 |
| R-factor | 0.1583 |
| Rwork | 0.157 |
| R-free | 0.18250 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.236 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXCD |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.350 | 1.950 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Number of reflections | 104897 | 5168 |
| <I/σ(I)> | 9.9 | 2.2 |
| Completeness [%] | 100.0 | 99.6 |
| Redundancy | 18 | 15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 0.1M sodium malonate, 12% w/v PEG3350 |
