5NXH
Crystal structure of the carboxy-terminal region of the bacteriophage T4 proximal long tail fibre protein gp34, residues 744-1289 at 2.9 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-13 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 107.297, 76.133, 139.868 |
| Unit cell angles | 90.00, 97.62, 90.00 |
Refinement procedure
| Resolution | 45.180 - 2.890 |
| R-factor | 0.2021 |
| Rwork | 0.200 |
| R-free | 0.26262 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5nxf |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.480 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.180 | 3.040 |
| High resolution limit [Å] | 2.890 | 2.890 |
| Rmerge | 0.098 | 0.436 |
| Number of reflections | 48930 | 6784 |
| <I/σ(I)> | 9.4 | 3 |
| Completeness [%] | 96.6 | 91.8 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 294 | 4-7% (w/v) PEG 6000 100 mM Tris-HCl pH 9.0 |
