5NX0
Structure of Spin-labelled T4 lysozyme mutant L115C-R119C-R1 at room temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2016-09-26 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 61.075, 61.075, 97.066 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 52.890 - 1.803 |
R-factor | 0.16044 |
Rwork | 0.158 |
R-free | 0.20853 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5lwo |
RMSD bond length | 0.019 |
RMSD bond angle | 1.879 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.890 | 1.868 |
High resolution limit [Å] | 1.803 | 1.803 |
Rmerge | 0.035 | 0.519 |
Number of reflections | 19432 | 1809 |
<I/σ(I)> | 13.92 | 1.12 |
Completeness [%] | 98.0 | 92 |
Redundancy | 1.8 | 1.6 |
CC(1/2) | 0.990 | 0.625 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.2 | 277 | 2.0 M K2HPO4/NAH2PO4, 250 MM NACL, 0.04 % AZIDE, 20 MM DITHIODIETHANOL, PH 7.2 |