5NWX
Insight into the molecular recognition mechanism of the coactivator NCoA1 by STAT6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.7 |
| Spacegroup name | P 62 |
| Unit cell lengths | 61.631, 61.631, 73.283 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 53.370 - 2.510 |
| R-factor | 0.18595 |
| Rwork | 0.184 |
| R-free | 0.23546 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oj5 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.948 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.370 | 2.610 |
| High resolution limit [Å] | 2.510 | 2.510 |
| Rmeas | 0.025 | 0.132 |
| Number of reflections | 5471 | |
| <I/σ(I)> | 30.26 | |
| Completeness [%] | 99.8 | 98.3 |
| Redundancy | 20.51 | 19.92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2 M sodium acetate, 0.1 M sodium cacodylate, pH 6.5, 30% PEG 8000, 5% PEG 400 |
