Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-11 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 208.477, 151.525, 195.279 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 195.280 - 3.760 |
| R-factor | 0.3161 |
| Rwork | 0.313 |
| R-free | 0.37050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yg2 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.780 | 3.890 |
| High resolution limit [Å] | 3.760 | 3.760 |
| Rmerge | 0.087 | 0.750 |
| Rpim | 0.031 | 0.356 |
| Number of reflections | 59333 | 5578 |
| <I/σ(I)> | 14.1 | 1.9 |
| Completeness [%] | 98.4 | 91.2 |
| Redundancy | 8.3 | 4.7 |
| CC(1/2) | 0.999 | 0.845 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.9 | 291 | 9% PEG8000, 18% Ethylene glycol, 100mM Sodium HEPES/MOPS Buffer, 0.03M NaF, 0.03M NaBr, 0.03M NaI, 0-10mM DTT |
