5NWJ
14-3-3c in complex with CPP7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-3 |
Synchrotron site | ESRF |
Beamline | MASSIF-3 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-02 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.96770 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 109.034, 109.034, 136.712 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.213 - 2.070 |
R-factor | 0.1965 |
Rwork | 0.194 |
R-free | 0.24200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1o9d |
RMSD bond length | 0.016 |
RMSD bond angle | 1.174 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.213 | 2.140 |
High resolution limit [Å] | 2.070 | 2.070 |
Rpim | 0.037 | 0.356 |
Number of reflections | 29779 | 2904 |
<I/σ(I)> | 18.3 | 5.1 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 40.9 | 39.8 |
CC(1/2) | 0.999 | 0.523 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 32% PEG 400, 0.2 M ammonium acetate pH 7.0, 0.1 M sodium citrate pH 4.4, 10 mM DTT and 5% glycerol |