5NWJ

14-3-3c in complex with CPP7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-02
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.96770
Spacegroup name	P 65 2 2
Unit cell lengths	109.034, 109.034, 136.712
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.213 - 2.070
R-factor	0.1965
Rwork	0.194
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1o9d
RMSD bond length	0.016
RMSD bond angle	1.174
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.213	2.140
High resolution limit [Å]	2.070	2.070
Rpim	0.037	0.356
Number of reflections	29779	2904
<I/σ(I)>	18.3	5.1
Completeness [%]	99.8	99.8
Redundancy	40.9	39.8
CC(1/2)	0.999	0.523

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	32% PEG 400, 0.2 M ammonium acetate pH 7.0, 0.1 M sodium citrate pH 4.4, 10 mM DTT and 5% glycerol