5NWF
Enterococcus faecalis FIC protein (H111A).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.980100 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 76.669, 77.107, 103.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.760 - 2.600 |
| R-factor | 0.194 |
| Rwork | 0.191 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5NUW |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.020 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 103.150 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.072 | 0.499 |
| Number of reflections | 19240 | |
| <I/σ(I)> | 15.5 | 3.6 |
| Completeness [%] | 99.1 | 99.9 |
| Redundancy | 5.9 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 50mM Tris pH 8.0, 200mM NaCl, 1mM MgCl2 0.2M Ammonium sulfate, 0.1M Tris-sodium citrate pH 5.6, 15% (w:v) PEG 4000 |
