5NWF
Enterococcus faecalis FIC protein (H111A).
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.980100 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 76.669, 77.107, 103.150 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 61.760 - 2.600 |
R-factor | 0.194 |
Rwork | 0.191 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5NUW |
RMSD bond length | 0.009 |
RMSD bond angle | 1.020 |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 103.150 | 2.720 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.072 | 0.499 |
Number of reflections | 19240 | |
<I/σ(I)> | 15.5 | 3.6 |
Completeness [%] | 99.1 | 99.9 |
Redundancy | 5.9 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 50mM Tris pH 8.0, 200mM NaCl, 1mM MgCl2 0.2M Ammonium sulfate, 0.1M Tris-sodium citrate pH 5.6, 15% (w:v) PEG 4000 |