5NVR
Crystal structure of the Rif1 N-terminal domain (RIF1-NTD) from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-22 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.97902 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 203.568, 203.568, 197.723 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.300 - 3.950 |
| R-factor | 0.214 |
| Rwork | 0.212 |
| R-free | 0.26100 |
| Structure solution method | MAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.541 |
| Data reduction software | DIALS (1.0-pre) |
| Data scaling software | Aimless (0.5.26) |
| Phasing software | SHARP (2.8.1) |
| Refinement software | PHENIX (DEV_2439) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.430 | 4.160 |
| High resolution limit [Å] | 3.940 | 3.940 |
| Rmerge | 0.234 | 4.131 |
| Number of reflections | 21894 | |
| <I/σ(I)> | 14.4 | 1.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 50.6 | 42.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 3.8 mg/ml protein solution in 50 mM HEPES pH 7.4, 310 mM NaCl, 1 mM TCEP mixed equally (1 uL + 1 uL) with 100 mM Tris-HCl, pH 7.5, 320 mM lithium sulfate, 850 mM potassium sodium tartrate. |
