5NVG
Thr12 Phosphorylated Ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-04 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97199 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 50.460, 24.740, 47.650 |
| Unit cell angles | 90.00, 113.17, 90.00 |
Refinement procedure
| Resolution | 43.810 - 1.070 |
| R-factor | 0.13092 |
| Rwork | 0.129 |
| R-free | 0.16653 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.037 |
| RMSD bond angle | 3.261 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.810 | 1.090 |
| High resolution limit [Å] | 1.070 | 1.070 |
| Rmerge | 0.087 | |
| Number of reflections | 22954 | |
| <I/σ(I)> | 7.5 | |
| Completeness [%] | 95.6 | |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 291.15 | 20% (v/v) ethanol, 20% (w/v) PEG-1000, 0.1M Phosphate-citrate pH 4.2 |
