5NV6
Structure of human transforming growth factor beta-induced protein (TGFBIp).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 61 |
| Unit cell lengths | 114.840, 114.840, 181.240 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.930 |
| R-factor | 0.22 |
| Rwork | 0.219 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vxp |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.500 | 3.110 |
| High resolution limit [Å] | 2.930 | 2.930 |
| Rmerge | 0.165 | 1.112 |
| Rmeas | 0.172 | 1.187 |
| Number of reflections | 28689 | 4338 |
| <I/σ(I)> | 17.1 | 1.9 |
| Completeness [%] | 98.5 | 90.8 |
| Redundancy | 13.1 | 8 |
| CC(1/2) | 0.997 | 0.580 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 4% polyethylene glycol 4,000 0.1 M sodium acetate, pH 4.6 |
