5NV5
Enterococcus faecalis FIC protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-3 |
Synchrotron site | ESRF |
Beamline | MASSIF-3 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-07 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.967700 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 121.541, 131.001, 136.935 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.330 - 2.400 |
R-factor | 0.167 |
Rwork | 0.164 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5NUW |
RMSD bond length | 0.010 |
RMSD bond angle | 1.080 |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 47.330 |
High resolution limit [Å] | 2.400 |
Number of reflections | 42957 |
<I/σ(I)> | 13.5 |
Completeness [%] | 99.7 |
Redundancy | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 10mM Hepes pH 7,4, 200mM NaCl, 50mM Bicine pH 8.4, 30% (w/v) PEG 2000 MME |