5NV5
Enterococcus faecalis FIC protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-07 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.967700 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 121.541, 131.001, 136.935 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.330 - 2.400 |
| R-factor | 0.167 |
| Rwork | 0.164 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5NUW |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.330 |
| High resolution limit [Å] | 2.400 |
| Number of reflections | 42957 |
| <I/σ(I)> | 13.5 |
| Completeness [%] | 99.7 |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 10mM Hepes pH 7,4, 200mM NaCl, 50mM Bicine pH 8.4, 30% (w/v) PEG 2000 MME |
