5NV1
SH3 domain from Mouse cortactin (C 2 2 21 crystal form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.007997 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 41.360, 55.950, 50.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.200 - 1.510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x69 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.200 | 1.580 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Rmerge | 0.070 | 0.831 |
| Number of reflections | 9248 | |
| <I/σ(I)> | 14.51 | |
| Completeness [%] | 98.3 | 96.5 |
| Redundancy | 6 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 1.6 M Mg-sulfate, 0.1 M MES pH 6.5 |
