5NUS

Structure of a minimal complex between p44 and p34 from Chaetomium thermophilum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-26
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97625
Spacegroup name	P 63 2 2
Unit cell lengths	147.056, 147.056, 87.599
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.418 - 2.200
R-factor	0.2106
Rwork	0.210
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.728
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.140	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.121	4.399
Rpim	0.029	1.133
Number of reflections	28834	2439
<I/σ(I)>	19.6	1
Completeness [%]	100.0	100
Redundancy	18.5	14.8
CC(1/2)	0.999	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	HEPES, PEG 4000, MPD