5NUQ
Structural basis for maintenance of bacterial outer membrane lipid asymmetry
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-09-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97886 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 115.491, 163.039, 230.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 94.242 - 3.200 |
R-factor | 0.2436 |
Rwork | 0.238 |
R-free | 0.30650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zfg |
RMSD bond length | 0.008 |
RMSD bond angle | 1.302 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 94.242 | 3.270 |
High resolution limit [Å] | 3.200 | 3.200 |
Rpim | 0.148 | 0.494 |
Number of reflections | 72631 | 4398 |
<I/σ(I)> | 4.5 | 1.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.6 | 7.5 |
CC(1/2) | 0.985 | 0.724 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.32 M lithium chloride 0.1M Sodium citrate pH 5.5 and 14% PEG 400 |