5NUF
Cytosolic Malate Dehydrogenase 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-26 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.541870 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 64.592, 118.545, 148.219 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.670 - 1.800 |
| Rwork | 0.190 |
| R-free | 0.31420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mdh |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.670 | 1.800 |
| High resolution limit [Å] | 1.800 | 0.864 |
| Rmerge | 0.172 | 1.072 |
| Rmeas | 0.186 | 1.252 |
| Rpim | 0.070 | 0.629 |
| Number of reflections | 110856 | |
| <I/σ(I)> | 10.15 | 1.3 |
| Completeness [%] | 94.4 | 67.49 |
| Redundancy | 6.7 | 3.7 |
| CC(1/2) | 0.985 | 0.454 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 283 | 0.18 M ammonium sulfate, 0.09 M sodium acetate trihydrate, 27% polyethylene glycol monomethyl ether 2000, 10% glycerol |
