5NUC
STAPHYLOCOCCAL NUCLEASE, 1-N-PENTANE THIOL DISULFIDE TO V23C VARIANT
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 278.15 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-01 |
| Detector | MACSCIENCE |
| Spacegroup name | P 41 |
| Unit cell lengths | 47.800, 47.800, 62.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.100 |
| R-factor | 0.169 |
| Rwork | 0.169 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1snc |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 6.000 | 2.190 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.060 | |
| Number of reflections | 6914 | |
| Completeness [%] | 87.4 | 85.6 |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8.15 | 4 * | Loll, P.J., (1989) Proteins Struct. Funct. Genet., 5, 183. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 10.5 (mM) | |
| 3 | 1 | drop | MPD | 17 (%(w/w)) | |
| 4 | 1 | drop | 10 (mM) | ||
| 5 | 1 | drop | potassium citrate | 20 (mM) | |
| 6 | 1 | reservoir | potassium phosphate | 10.5 (mM) | |
| 7 | 1 | reservoir | MPD | 21-23 (%(w/w)) |
