5NU8
Structure of human urine RBP4 saturated with palmitate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-18 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 |
| Unit cell lengths | 102.790, 102.790, 73.634 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.590 |
| R-factor | 0.17203 |
| Rwork | 0.170 |
| R-free | 0.21167 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5nu2 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.643 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.740 | 1.680 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.053 | 0.466 |
| Number of reflections | 35401 | |
| <I/σ(I)> | 9.2 | 2 |
| Completeness [%] | 90.9 | 92.3 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 4.3 M NaCl, 100 mM Hepes, pH 7.5 |
