5NU7
Structure of human holo plasma RBP4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-14 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 |
| Unit cell lengths | 103.097, 103.097, 73.552 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.500 |
| R-factor | 0.18114 |
| Rwork | 0.180 |
| R-free | 0.20960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1brp |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.641 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.160 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.058 | 0.462 |
| Number of reflections | 46674 | |
| <I/σ(I)> | 12.6 | 2.8 |
| Completeness [%] | 99.5 | 99.4 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 277 | 4.5 M NaCl, 20 mM Na cacodylate, pH 6.8 |
