5NTY
Structure of non-fluorescent human plasma RBP4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-16 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 |
| Unit cell lengths | 103.770, 103.770, 73.940 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.17501 |
| Rwork | 0.172 |
| R-free | 0.23031 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1brq |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.605 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.106 | 0.491 |
| Number of reflections | 19780 | |
| <I/σ(I)> | 7.9 | 2.5 |
| Completeness [%] | 98.5 | 98.3 |
| Redundancy | 4.4 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 279 | 4.5M NaCl, 20mM Na cacodylate, pH 6.8 |
