5NT1
Complex of influenza A NS1 effector domain with TRIM25 coiled coil
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-01 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 131.380, 225.230, 146.430 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.685 - 2.820 |
R-factor | 0.1964 |
Rwork | 0.195 |
R-free | 0.22440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ltb |
RMSD bond length | 0.006 |
RMSD bond angle | 0.800 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.680 | 2.890 |
High resolution limit [Å] | 2.820 | 2.820 |
Rmerge | 0.092 | |
Rpim | 0.042 | |
Number of reflections | 52603 | 3878 |
<I/σ(I)> | 14.9 | 1.4 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.8 | |
CC(1/2) | 1.000 | 0.600 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 100 mM Tris pH 8.5 25 % PEG 600 200 mM sodium citrate |