5NST
Human monoclonal antibody with a LAIR1 insertion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-01-27 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.92 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 169.823, 86.462, 103.998 |
Unit cell angles | 90.00, 126.65, 90.00 |
Refinement procedure
Resolution | 81.900 - 2.520 |
R-factor | 0.2214 |
Rwork | 0.219 |
R-free | 0.26730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3KGR and 3DIF |
RMSD bond length | 0.010 |
RMSD bond angle | 1.230 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 81.900 |
High resolution limit [Å] | 2.520 |
Number of reflections | 40863 |
<I/σ(I)> | 7.4 |
Completeness [%] | 99.8 |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 20% PEG 4000, 0.1M sodium citrate pH 4.5 |