5NSG

Fc DEDE homodimer variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-01-28
Detector	MAR CCD 165 mm
Wavelength(s)	0.87291
Spacegroup name	C 2 2 21
Unit cell lengths	50.000, 147.010, 76.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.816 - 2.220
R-factor	0.2008
Rwork	0.198
R-free	0.24310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4cdh
RMSD bond length	0.006
RMSD bond angle	0.833
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11_2567: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.820	2.290
High resolution limit [Å]	2.220	2.220
Rmerge	0.052	0.250
Rpim	0.032	0.159
Number of reflections	14198	1264
<I/σ(I)>	15.9	4.5
Completeness [%]	99.2	98.4
Redundancy	3.4	3.3
CC(1/2)	0.998	0.916

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	291	acetate buffer pH 4.5, 0.2 M zinc acetate, 16% w/v PEG 3000