5NS7
Crystal structure of beta-glucosidase BglM-G1 mutant H75R from marine metagenome
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.00238 |
Spacegroup name | P 43 |
Unit cell lengths | 137.920, 137.920, 98.040 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.762 - 1.540 |
R-factor | 0.1592 |
Rwork | 0.159 |
R-free | 0.17170 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 0.955 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | PHENIX (dev_2621) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.762 | 48.762 | 1.580 |
High resolution limit [Å] | 1.540 | 6.890 | 1.540 |
Rmerge | 0.104 | 0.039 | 1.821 |
Rmeas | 0.112 | 0.042 | 1.992 |
Total number of observations | 1840750 | ||
Number of reflections | 270326 | 3131 | 19952 |
<I/σ(I)> | 11.28 | 42.62 | 0.89 |
Completeness [%] | 99.9 | 99.6 | 99.7 |
Redundancy | 6.809 | 7.333 | 6.157 |
CC(1/2) | 0.998 | 0.998 | 0.315 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7 | 291 | 0.1M Na-HEPES, 2M AmmSO4, 2% PEG400 |