5NS4
Crystal structures of Cy3 cyanine fluorophores stacked onto the end of double-stranded RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2013-06-22 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979601 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 93.757, 122.432, 51.013 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.880 - 2.400 |
R-factor | 0.261 |
Rwork | 0.260 |
R-free | 0.28300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mji |
RMSD bond length | 0.013 |
RMSD bond angle | 1.531 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.880 | 2.486 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.100 | 0.162 |
Rmeas | 0.114 | 0.183 |
Number of reflections | 23654 | 2309 |
<I/σ(I)> | 12.48 | 6.26 |
Completeness [%] | 100.0 | 100 |
Redundancy | 4.4 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 50 mM sodium cacodylate at pH 6.5, 100 mM Mg(CH3COO)2, 50 mM KF, 15% PEG 8000 drop |