5NS3

Crystal structures of Cy5 cyanine fluorophores stacked onto the end of double-stranded RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	94
Detector technology	PIXEL
Collection date	2014-02-25
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1
Spacegroup name	P 21 21 2
Unit cell lengths	95.043, 123.895, 51.201
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.521 - 2.400
R-factor	0.253
Rwork	0.252
R-free	0.27450
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.520	2.058
High resolution limit [Å]	1.987	1.987
Rmerge	0.076	1.275
Rmeas	0.083	1.386
Number of reflections	42321	4055
<I/σ(I)>	15.77	1.44
Completeness [%]	99.0	97
Redundancy	6.6	6.5
CC(1/2)	0.995	0.561

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		289	0.3 M Magnesium formate dehydrate 0.1 M BIS-TRIS pH 5.5 50mM KF