5NS3
Crystal structures of Cy5 cyanine fluorophores stacked onto the end of double-stranded RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 94 |
Detector technology | PIXEL |
Collection date | 2014-02-25 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 95.043, 123.895, 51.201 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.521 - 2.400 |
R-factor | 0.253 |
Rwork | 0.252 |
R-free | 0.27450 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.520 | 2.058 |
High resolution limit [Å] | 1.987 | 1.987 |
Rmerge | 0.076 | 1.275 |
Rmeas | 0.083 | 1.386 |
Number of reflections | 42321 | 4055 |
<I/σ(I)> | 15.77 | 1.44 |
Completeness [%] | 99.0 | 97 |
Redundancy | 6.6 | 6.5 |
CC(1/2) | 0.995 | 0.561 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 289 | 0.3 M Magnesium formate dehydrate 0.1 M BIS-TRIS pH 5.5 50mM KF |