5NRG

The crystal structure of the large ribosomal subunit of Staphylococcus aureus in complex with RB02

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.971
Spacegroup name	P 65 2 2
Unit cell lengths	279.797, 279.797, 873.274
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.369 - 3.442
R-factor	0.221
Rwork	0.219
R-free	0.26130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wce
RMSD bond length	0.009
RMSD bond angle	1.390
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.510
High resolution limit [Å]	3.442	9.350	3.450
Rmerge	0.188	0.103	0.772
Rmeas	0.196	0.107	0.868
Rpim	0.052	0.028	0.375
Number of reflections	254888
<I/σ(I)>	5.5
Completeness [%]	96.3	96.9	72.8
Redundancy	11.2	12.4	3.6
CC(1/2)		0.994	0.640

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	1:2 MPD: EtOH, Hepes pH=7.6 10mM, Mg2Cl 10mM, KCl 60mM, NH4Cl 15mM