5NQT

Tubulin Darpin room-temperature structure determined by serial millisecond crystallography

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	293
Detector technology	PIXEL
Collection date	2016-05-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	74.040, 91.430, 83.610
Unit cell angles	90.00, 96.82, 90.00

Refinement procedure

Resolution	33.600 - 2.150
R-factor	0.182
Rwork	0.179
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4drx
RMSD bond length	0.010
RMSD bond angle	1.060
Data reduction software	CrystFEL (0.6.2)
Data scaling software	CrystFEL (0.6.2)
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.100	2.210
High resolution limit [Å]	2.130	2.130
Number of reflections	65679	6535
<I/σ(I)>	6.56	0.74
Completeness [%]	100.0	100
Redundancy	395.9	21.8
CC(1/2)	0.990	0.190

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	20-24% PEG 3350, 0.2 M Ammonium Sulfate, 0.1 M Bis-Tris Methane pH5.5