5NQT
Tubulin Darpin room-temperature structure determined by serial millisecond crystallography
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2016-05-30 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.040, 91.430, 83.610 |
Unit cell angles | 90.00, 96.82, 90.00 |
Refinement procedure
Resolution | 33.600 - 2.150 |
R-factor | 0.182 |
Rwork | 0.179 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4drx |
RMSD bond length | 0.010 |
RMSD bond angle | 1.060 |
Data reduction software | CrystFEL (0.6.2) |
Data scaling software | CrystFEL (0.6.2) |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.100 | 2.210 |
High resolution limit [Å] | 2.130 | 2.130 |
Number of reflections | 65679 | 6535 |
<I/σ(I)> | 6.56 | 0.74 |
Completeness [%] | 100.0 | 100 |
Redundancy | 395.9 | 21.8 |
CC(1/2) | 0.990 | 0.190 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 20-24% PEG 3350, 0.2 M Ammonium Sulfate, 0.1 M Bis-Tris Methane pH5.5 |