5NQQ
Rabbit Muscle L-lactate dehydrogenase in complex with NADH and oxaloacetate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-13 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.373, 138.763, 74.567 |
| Unit cell angles | 90.00, 110.12, 90.00 |
Refinement procedure
| Resolution | 29.416 - 1.872 |
| R-factor | 0.1715 |
| Rwork | 0.170 |
| R-free | 0.19900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h3f |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.227 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.420 |
| High resolution limit [Å] | 1.872 |
| Number of reflections | 220343 |
| <I/σ(I)> | 14 |
| Completeness [%] | 98.9 |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 10% wt/v PEG 3350, 50 mM Bis-tris propane pH 6.5 and 100 mM NaF |
