5NQP
Structure of a fHbp(V1.4):PorA(P1.16) chimera. Fusion at fHbp position 151.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-16 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 100.720, 171.420, 131.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.745 - 2.860 |
| R-factor | 0.2003 |
| Rwork | 0.198 |
| R-free | 0.23910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ayd |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.584 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.750 | 2.930 |
| High resolution limit [Å] | 2.860 | 2.860 |
| Rmerge | 0.074 | 0.645 |
| Rpim | 0.038 | 0.340 |
| Number of reflections | 26403 | 1923 |
| <I/σ(I)> | 17.1 | 2 |
| Completeness [%] | 99.1 | 99.4 |
| Redundancy | 4.5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 294 | 0.15M sodium citrate, 2.0M ammonium sulphate |
