5NQP

Structure of a fHbp(V1.4):PorA(P1.16) chimera. Fusion at fHbp position 151.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-16
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97949
Spacegroup name	C 2 2 21
Unit cell lengths	100.720, 171.420, 131.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.745 - 2.860
R-factor	0.2003
Rwork	0.198
R-free	0.23910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ayd
RMSD bond length	0.002
RMSD bond angle	0.584
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.750	2.930
High resolution limit [Å]	2.860	2.860
Rmerge	0.074	0.645
Rpim	0.038	0.340
Number of reflections	26403	1923
<I/σ(I)>	17.1	2
Completeness [%]	99.1	99.4
Redundancy	4.5	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	294	0.15M sodium citrate, 2.0M ammonium sulphate